Protein_Domain

Part:BBa_K105003:Design

Designed by: Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-12)

tetR - DNA binding domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction sites and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> <br\n> The frist codon has been changed from TCC to AGT (both Ser) to prevent a methylation site. <br\n> <br\n> <br\n>

For more information about this issus, see:<br\n>

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

We changed the format of the TetR coding part of BBa_C0040 into Phillips/Silver standard.

References